The Phospho-HSL-S853 Monoclonal Antibody (CABP1151) is a high-quality antibody developed for reliable detection and analysis of target proteins. HSL is a key enzyme involved in the regulation of lipid metabolism, particularly in the breakdown of stored fats into fatty acids and glycerol.This antibody, raised in rabbits, is highly specific and sensitive for detecting phosphorylated HSL at serine 853 in human samples. It has been validated for use in Western blot applications, allowing for precise analysis of HSL phosphorylation levels in various cell types.Phosphorylation of HSL at serine 853 is known to play a crucial role in the regulation of lipid metabolism and energy homeostasis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
Phospho-HSL-S853 Monoclonal Antibody
SKU:
CABP1151
Size:
20μL, 100μL
Reactivity:
Mouse
Clone Number:
ARC1657
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
Email for sequence
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids.
Purification Method
Affinity purification
Gene ID
3991
RRID
AB_2864015
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-HSL-S853 Rabbit mAb (CABP1151) at 1:1000 dilution. NIH/3T3 cells were treated with Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
