The Phospho-HSP27/HSPB1-S82 Monoclonal Antibody (CABP1031) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity in detecting phosphorylated Hsp27 (S82) in human samples, and has been validated for use in Western blot applications.Hsp27, also known as heat shock protein 27, is a chaperone protein that plays a crucial role in protecting cells from stress-induced damage and promoting cell survival.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
Phospho-HSP27/HSPB1-S82 Monoclonal Antibody
SKU:
CABP1031
Size:
20μL, 100μL
Reactivity:
Human, Rat
Clone Number:
ARC1576
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
QLSS G
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
HeLa treated with UV, PC-12, PC-12 treated with UV
Cellular Localization:
Cytoplasm, Nucleus, Cytoskeleton, Spindle.
Calculated MW:
23kDa
Observed MW:
27kDa
This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy.
Purification Method
Affinity purification
Gene ID
3315
RRID
AB_2863914
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from PC-12 cells, using Phospho-HSP27/HSPB1-S82 Rabbit mAb (CABP1031) at 1:1000 dilution. PC-12 cells were treated with CIP (1 U/ul) at 37℃ for 1 hour or treated with UV at room temperature for 15-30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Western blot analysis of lysates from HeLa cells, using Phospho-HSP27/HSPB1-S82 Rabbit mAb (CABP1031) at 1:1000 dilution. HeLa cells were treated with UV at room temperature for 15-30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
