The Phospho-Lamin A/C-S22 Antibody (CABP0777) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, raised in rabbits, is highly specific for detecting phosphorylated LMNA at serine 22 in human samples and is validated for use in Western blot applications.Phosphorylation of LMNA at serine 22 has been implicated in various cellular processes, including cell cycle regulation, DNA replication, and chromatin organization. Dysregulation of LMNA phosphorylation has been linked to diseases such as progeria, muscular dystrophy, and cancer.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
Phospho-Lamin A/C-S22 Antibody
SKU:
CABP0777
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
PLSP T
Tested Applications:
WBIPELISA
Recommended Dilution:
WB
1:500 - 1:2000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
The protein encoded by this gene is part of the nuclear lamina, a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.
Purification Method
Affinity purification
Gene ID
4000
RRID
AB_2771274
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-Lamin A/C-S22 Rabbit pAb (CABP0777) at 1:2000 dilution or Lamin A antibody (CAB0249). HeLa cells were treated with Hydroxyurea (4 mM) at 37℃ for 20 hours or treated with Paclitaxel (100 nM/mL) at 37℃ for 20 hours. C6 cells were treated with Hydroxyurea (4 mM) at 37℃ for 20 hours or treated with Paclitaxel (100 nM) at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit (). Exposure time: 1s.
Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg Phospho-Lamin A/C-S22 pAb (CABP0777). Western blot was performed from the immunoprecipitate using Phospho-Lamin A/C-S22 pAb (CABP0777) at a dilution of 1:1000. HeLa cells were treated with Paclitaxel (100 nM/mL) at 37℃ for 20 hours.
