The Phospho-MAP2K1-S217/MAP2K2-S221 Antibody (CABP0209) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for phosphorylated MAP2K1 at Serine 217 and MAP2K2 at Serine 221 in human samples, making it ideal for Western blot applications.Activation of MAP2K1 and MAP2K2 has been linked to various cellular processes, including cell proliferation, differentiation, and apoptosis. Phosphorylation at specific sites, such as Serine 217 and Serine 221, is crucial for their activation and downstream signaling cascades.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-MAP2K1-S217/MAP2K2-S221 Antibody
SKU:
CABP0209
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
SMAN S
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:2000 - 1:7000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MEK1/MEK2, Phospho-MAP2K1-S217/MAP2K2-S221
Positive Sample:
HeLa treated with PMA
Cellular Localization:
Cytosol, Early Endosome, Endoplasmic Reticulum, Focal Adhesion, Golgi Apparatus, Late Endosome, Microtubule Organizing Center, Mitochondrion, Nucleus, Plasma Membrane.
Calculated MW:
40kDa/43kDa/44kDa
Observed MW:
44kDa
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]
Purification Method
Affinity purification
Gene ID
5604 5605
RRID
AB_2771278
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells using Phospho-MAP2K1-S217/MAP2K2-S221 Rabbit pAb (CABP0209) at 1:6000 dilution. HeLa cells were treated with PMA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
