The MEK7 Monoclonal Antibody (CAB12950) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is specifically designed for use in immunohistochemistry, western blotting, and flow cytometry, offering high sensitivity and specificity in detecting MEK7 expression in various cell types and tissues.MEK7, also known as MAP2K7, is a crucial component of the MAPK pathway that mediates cellular responses to extracellular signals, making it a promising target for studying diseases such as cancer, inflammatory disorders, and neurodegenerative diseases.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MEK7 Monoclonal Antibody
SKU:
CAB12950
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0712
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically activates MAPK8/JNK1 and MAPK9/JNK2, and this kinase itself is phosphorylated and activated by MAP kinase kinase kinases including MAP3K1/MEKK1, MAP3K2/MEKK2,MAP3K3/MEKK5, and MAP4K2/GCK. This kinase is involved in the signal transduction mediating the cell responses to proinflammatory cytokines, and environmental stresses. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
5609
RRID
AB_2861679
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse brain using [KO Validated] MEK7 Rabbit mAb (CAB12950) at 1:1000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90 s.
Western blot analysis of lysates from wild type(WT) and MEK7 knockout (KO) 293T cells using [KO Validated] MEK7 Rabbit mAb (CAB12950) at 1:1000 dilution incubated overnight 4 ℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30 s.
