The Phospho-MYL9-S19 Antibody (CABP0872) is a high-quality antibody developed for reliable detection and analysis of target proteins. Myosin regulatory light chain 9 (MyL9) is a key regulatory protein involved in actomyosin contractility and cell motility. This antibody specifically recognizes phosphorylated MyL9 at serine 19, enabling the detection and analysis of phosphorylated MyL9 in a variety of cell types.Raised in rabbits, this antibody is highly reactive with human samples and has been validated for use in Western blot applications. By targeting the phosphorylated form of MyL9, researchers can investigate the regulation of actomyosin contractility in different cellular contexts.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-MYL9-S19 Antibody
SKU:
CABP0872
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
ATSN V
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:100 - 1:500
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Cell Cortex, Cytoplasm, Cytosol, Myofibril, Z Disc.
Calculated MW:
20kDa
Observed MW:
18kDa
Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
10398
RRID
AB_2771352
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from C2C12 cells, using Phospho-MYL9-S19 Rabbit pAb (CABP0872) at 1:400 dilution. C2C12 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
