The Phospho-NR3C1 (S211) Polyclonal Antibody (CABP0759) is a valuable tool for researchers studying the NR3C1 protein, also known as the glucocorticoid receptor. This antibody, produced in rabbits, is highly sensitive and specific for detecting phosphorylation at serine 211 of the NR3C1 protein in human samples. Validated for use in Western blot applications, this antibody enables accurate detection and quantification of phosphorylated NR3C1 in various cell types.The NR3C1 protein is a key regulator of cellular response to stress and inflammation, and its phosphorylation at serine 211 plays a crucial role in modulating its activity.
Research into the phosphorylation status of NR3C1 is important for understanding its role in various physiological processes, including immune response, metabolism, and cell growth. This antibody provides researchers with a reliable tool for investigating the impact of NR3C1 phosphorylation on gene expression and cellular signaling pathways, making it a valuable asset for studies in immunology, cancer research, and endocrinology.
This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175).
Purification Method:
Affinity purification
Gene ID:
2908
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of NIH/3T3, using Phospho-Glucocorticoid Receptor-S211 Rabbit pAb (CABP0759) at 1:600 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.
