The Phospho-NRF2-S40 Polyclonal Antibody (CABP1167) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly reactive with human samples and is validated for use in various applications including Western blot and immunohistochemistry.NRF2 is a transcription factor that plays a crucial role in cellular defense against oxidative stress and inflammation by regulating the expression of antioxidant and detoxifying genes. Phosphorylation of NRF2 at serine 40 is known to enhance its stability and transcriptional activity, making it an important post-translational modification for NRF2 function.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-NRF2-S40 Polyclonal Antibody
SKU:
CABP1167
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NRF2, HEBP1, Nrf-2, IMDDHH, Phospho-NRF2-S40
Cellular Localization:
Cytoplasm, Nucleus, Cytosol.
Calculated MW:
68kDa
Observed MW:
Refertofigures
This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene.
Purification Method
Affinity purification
Gene ID
4780
RRID
AB_2864026
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Immunofluorescence analysis of Hep G2 cells using Phospho-NRF2-S40 Rabbit pAb (CABP1167) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
