The Phospho-PDGFRA-Y849 Antibody (CABP0569) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in applications such as Western blotting.The phosphorylation of PDGFRα at tyrosine 849 plays a key role in the activation of downstream signaling pathways involved in cell proliferation, migration, and survival. Research into the regulation of this phosphorylation event is important in understanding the mechanisms underlying diseases such as cancer, where dysregulation of PDGFRα signaling can drive tumor growth and metastasis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-PDGFRA-Y849 Antibody
SKU:
CABP0569
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
SNYV S
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CD140A, PDGFR2, PDGFR-2, Phospho-PDGFR alpha-Y849
Positive Sample:
Hep G2 treated with EGF
Cellular Localization:
Cell Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
123kDa
Observed MW:
190kDa
This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development, wound healing, and tumor progression. Mutations in this gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.
Purification Method
Affinity purification
Gene ID
5156
RRID
AB_2771404
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HepG2 cells, using Phospho-PDGFR alpha-Y849 Rabbit pAb (CABP0569) at 1:1000 dilution. HepG2 cells were treated with EGF (100ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA.
