The Phospho-PFKFB2-S483 Antibody (CABP0784) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific to the phosphorylated form of PFKFB2 at serine 483 and is validated for use in Western blot applications. By detecting and quantifying the phosphorylation status of PFKFB2, researchers can gain insights into its role in metabolic pathways and potential implications in diseases such as cancer, diabetes, and metabolic disorders.PFKFB2 is a key regulator of glycolysis, the process by which cells convert glucose into energy. Phosphorylation of PFKFB2 at serine 483 can modulate its enzymatic activity, impacting glycolytic flux and cellular metabolism.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-PFKFB2-S483 Antibody
SKU:
CABP0784
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
NYSV G
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PFK-2/FBPase-2, Phospho-PFKFB2-S483
Positive Sample:
HeLa treated with IGF-1
Cellular Localization:
Cytosol, Nucleoplasm.
Calculated MW:
58kDa
Observed MW:
55kDa
The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
5208
RRID
AB_2771414
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-PFKFB2-S483 Rabbit pAb (CABP0784) at 1:2000 dilution. HeLa cells were treated with IGF-1 (50 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
