The Phospho-PIK3R1/PIK3R2/PIK3R3-Y467 Polyclonal Antibody (CABP0427) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is optimized for detection in human samples and is suitable for Western blot applications. By targeting the phosphorylation site at tyrosine 467, this antibody allows for precise analysis of PI3K signaling in various cell types.The PI3K pathway is a key regulator of cell growth, survival, and metabolism, with dysregulation of this pathway implicated in numerous diseases including cancer, diabetes, and cardiovascular disorders.
This antibody is validated for use in WB applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Cell-Cell Junction, Cis-Golgi Network, Cytoplasm, Cytosol, Nucleus, Perinuclear Region Of Cytoplasm, Plasma Membrane.
Calculated MW:
42-54kDa/81-84kDa
Observed MW:
85kDa
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
Purification Method
Affinity purification
Gene ID
5295 5296 8503
RRID
AB_2771417
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from 293 cells, using Phospho-PI3KP85α/P55γ/P85β-Y467/Y464/Y199 Rabbit pAb (CABP0427). Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA.
