The Phospho-PKC mu-S910 Polyclonal Antibody (CABP1380) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, has high specificity for human samples and is suitable for use in Western blot applications. By binding to the phosphorylated form of PKC mu, this antibody enables the detection and analysis of this critical signaling molecule in various cell types.PKC mu is a member of the protein kinase C family, known for its role in regulating various cellular processes, including cell growth, differentiation, and apoptosis. Phosphorylation of PKC mu at serine 910 has been linked to its activation and involvement in signaling pathways related to cell proliferation and survival.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-PKC mu-S910 Polyclonal Antibody
SKU:
CABP1380
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
The protein encoded by this gene is a serine/threonine protein kinase involved in many cellular processes, including Golgi body membrane integrity and transport, cell migration and differentiation, MAPK8/JNK1 and Ras pathway signaling, MAPK1/3 (ERK1/2) pathway signaling, cell survival, and regulation of cell shape and adhesion.
Purification Method
Affinity purification
Gene ID
5587
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from 293F cells using Phospho-PKC mu-S910 Rabbit pAb (CABP1380) at 1:4000 dilution. 293F cells were treated with PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
