The Phospho-PKC alpha-S319 Antibody (CABP0560) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity for human samples and has been validated for use in Western blot applications.PKCalpha is a member of the protein kinase C (PKC) family, which plays a key role in various cellular processes, including cell growth, differentiation, and apoptosis. Phosphorylation of PKCalpha at Serine 319 has been implicated in the regulation of its enzymatic activity and signaling pathways.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-PKC alpha-S319 Antibody
SKU:
CABP0560
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
VISP S
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.
Purification Method
Affinity purification
Gene ID
5578
RRID
AB_2771422
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-PKC alpha-S319 Rabbit pAb (CABP0560) at 1:1000 dilution. HeLa cells were treated with PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight or treated with ATP(5 mM) at 30℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
