The Phospho-PLC gamma 2 (PLCG2)-Y1217 Antibody (CABP0805) is a high-quality antibody developed for reliable detection and analysis of target proteins. This phosphorylation event is known to activate PLCG2, a crucial enzyme involved in intracellular signaling pathways regulating immune responses and cell proliferation.Raised in rabbits, this antibody is highly specific to human samples and has been validated for use in Western blot applications. By specifically binding to the phosphorylated form of PLCG2 at tyrosine 1217, researchers can accurately detect and analyze this key protein modification in various cell types.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Phospho-PLC gamma 2 (PLCG2)-Y1217 Antibody
SKU:
CABP0805
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
FLYD T
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Cytoplasm, Cytosol, Extracellular Exosome, Perinuclear Region Of Cytoplasm, Plasma Membrane, Ruffle Membrane.
Calculated MW:
148kDa
Observed MW:
150kDa
The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.
Purification Method
Affinity purification
Gene ID
5336
RRID
AB_2771437
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Raji cells, using Phospho-PLC gamma 2 (PLCG2)-Y1217 Rabbit pAb (CABP0805) at 1:1000 dilution or PLC gamma 2 (PLCG2) antibody (CAB2182). Raji cells were treated with Pervanadate (1 mM) at 37℃ for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Phospho-PLC gamma 2 (PLCG2)-Y1217 Rabbit pAb (CABP0805) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
