The Phospho-POLR2A CTD-T4 Polyclonal Antibody (CAB21190) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, specifically targets the phosphorylated form of POLR2A C-terminal domain (CTD), a key player in RNA synthesis and processing. Validated for use in Western blot and immunohistochemistry applications, this antibody is highly reactive with human samples, allowing for precise detection and analysis of phospho-POLR2A in various cell types. Its specificity for the phosphorylated form of POLR2A makes it an ideal choice for studies in gene expression regulation, RNA splicing, and transcriptional control mechanisms.
This antibody is validated for use in WB, IP, ChIP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-POLR2A CTD-T4 Polyclonal Antibody
SKU:
CAB21190
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBIPChIPELISA
Recommended Dilution:
WB
1:500 - 1:1000
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
Purification Method
Affinity purification
Gene ID
5430
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Phospho-POLR2A CTD-T4 Rabbit pAb (CAB21190) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunoprecipitation analysis of 300 μg extracts of C6 cells using 3 μg Phospho-POLR2A CTD-T4 antibody (CAB21190). Western blot was performed from the immunoprecipitate using Phospho-POLR2A (Thr4) antibody (CAB21190) at a dilution of 1:1000.
