The Phospho-PPP1R12A-S668 Antibody (CABP0835) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for detecting the phosphorylated form of PPP1R12A in human samples and is validated for use in Western blot applications.PPP1R12A is a key regulatory subunit of protein phosphatase 1 (PP1) that plays a crucial role in the regulation of actomyosin contractility. Phosphorylation of PPP1R12A at serine 668 is known to modulate its activity, impacting processes such as smooth muscle contraction and cell migration. The Phospho-PPP1R12A (S668) Polyclonal Antibody allows for the detection and quantification of this phosphorylation event, facilitating research into the signaling pathways and biological functions regulated by PPP1R12A.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-PPP1R12A-S668 Antibody
SKU:
CABP0835
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
RRSY L
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MBS, GUBS, M130, MYPT1, Phospho-PPP1R12A-S668
Positive Sample:
HeLa treated with Calyculin A, C6 treated with Calyculin A
Cellular Localization:
Cytoplasm.
Calculated MW:
115kDa
Observed MW:
160kDa
Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
4659
RRID
AB_2771448
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-PPP1R12A-S668 Rabbit pAb (CABP0835) at 1:2000 dilution. Both HeLa cells and C6 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
