The Phospho-AMPKalpha1-S485 Antibody (CABP0871) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. RRID AB_2771450 Gene ID 5562 Swiss Prot Synonym AMPK; AMPKa1; AMPK alpha 1; Phospho-AMPKα1-S485
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-AMPKalpha1-S485 Antibody
SKU:
CABP0871
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AMPK, AMPKa1, AMPK alpha 1, Phospho-AMPKα1-S485
Positive Sample:
C6
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
64kDa
Observed MW:
62kDa
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. RRID AB_2771450 Gene ID 5562 Swiss Prot Synonym AMPK; AMPKa1; AMPK alpha 1; Phospho-AMPKα1-S485
Purification Method:
Affinity purification
Gene ID:
5562
RRID:
AB_2771450
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells using Phospho-AMPKα1-S485 Rabbit pAb (CABP0871) at 1:2000 dilution. C6 cells were treated by λpp(20uL/400ul) at 37℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
