The PKC delta Antibody (CAB0471) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, derived from rabbits, is highly specific to human samples and has been validated for use in Western blot applications.PRKCD, also known as protein kinase C delta, is a crucial player in signaling pathways that regulate cell proliferation and survival. Dysregulation of PRKCD has been linked to diseases such as cancer, diabetes, and cardiovascular disorders, making it a promising target for therapeutic interventions.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PKC delta Antibody
SKU:
CAB0471
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrome.
Purification Method
Affinity purification
Gene ID
5580
RRID
AB_2757209
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PKC delta Rabbit pAb (CAB0471) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1min.
Immunohistochemistry analysis of paraffin-embedded Rat lung using PKC delta Rabbit pAb (CAB0471) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse testis using PKC delta Rabbit pAb (CAB0471) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of NIH/3T3 cells using PKC delta Rabbit pAb (CAB0471) at dilution of 1:100. Blue: DAPI for nuclear staining.
