The PTEN Rabbit Polyclonal Antibody is a valuable tool for researchers studying PTEN, a tumor suppressor gene that plays a crucial role in cell growth and division. This antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to the PTEN protein, this antibody enables accurate detection and analysis in various cell types, making it ideal for studies in cancer research and molecular biology.PTEN, also known as phosphatase and tensin homolog, is a key regulator of cell survival and proliferation.
Mutations in the PTEN gene are associated with a variety of cancers, making it a significant target for therapeutic interventions. Understanding the function of PTEN is essential for developing novel treatments that target cancer progression and metastasis. This antibody provides researchers with a reliable tool for investigating the role of PTEN in cancer biology and identifying potential therapeutic strategies.
Product Name:
PTEN Rabbit Polyclonal Antibody
SKU:
CAB0008
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PTEN (NP_000305.3).
This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
5728
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using PTEN Rabbit pAb (CAB0008) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
