Description
Phospho-Rac1/Cdc42-S71 Monoclonal Antibody (CABP1018)
The Phospho-Rac1/Cdc42-S71 Monoclonal Antibody (CABP1018) is a high-quality antibody developed for reliable detection and analysis of target proteins. These proteins are key regulators of cell migration, cytoskeletal organization, and cell proliferation. The antibody is produced in rabbits and is highly specific for detecting phosphorylation at serine 71 of Rac1 and Cdc42 in human samples.This antibody is validated for use in Western blot applications, providing researchers with a reliable method to study the activation status of Rac1 and Cdc42 in different cell types. Understanding the phosphorylation of these proteins is essential for investigating their roles in cellular processes such as cell adhesion, migration, and invasion.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
| Product Name: | Phospho-Rac1/Cdc42-S71 Monoclonal Antibody |
| SKU: | CABP1018 |
| Size: | 20μL, 100μL |
| Reactivity: | Human |
| Clone Number: | ARC1563 |
| Conjugate: | Unconjugated |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. | ||||
| Sequence: | PLSY P | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
| ||||
| Synonyms: | CDC42Hs, G25K, TKS, Phospho-Rac1/Cdc42-S71 |
| Positive Sample: | A-431 |
| Cellular Localization: | Cytoplasm, Cytoplasmic Ribonucleoprotein Granule, Cytosol, Dendritic Spine, Extracellular Exosome, Filopodium, Focal Adhesion, Golgi Transport Complex, Leading Edge Membrane, Microtubule Organizing Center, Mitotic Spindle, Phagocytic Vesicle, Plasma Membrane, Spindle Midzone. |
| Calculated MW: | 24kDa |
| Observed MW: | 24kDa |
The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. [provided by RefSeq, Apr 2013]
| Purification Method | Affinity purification |
| Gene ID | 998 5879 |
| RRID | AB_2863904 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of lysates from A-431 cells, using Phospho-Rac1/Cdc42-S71 Rabbit mAb (CABP1018) at 1:1000 dilution. A-431 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min. |
