The Phospho-Rac1/Cdc42-S71 Monoclonal Antibody (CABP1018) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. [provided by RefSeq, Apr 2013]
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-Rac1/Cdc42-S71 Monoclonal Antibody
SKU:
CABP1018
Size:
100μL, 20μL
Reactivity:
Human
Clone Number:
ARC1563
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CDC42Hs, G25K, TKS, Phospho-Rac1/Cdc42-S71
Positive Sample:
A-431
Cellular Localization:
Cytoplasm, Cytoplasmic Ribonucleoprotein Granule, Cytosol, Dendritic Spine, Extracellular Exosome, Filopodium, Focal Adhesion, Golgi Transport Complex, Leading Edge Membrane, Microtubule Organizing Center, Mitotic Spindle, Phagocytic Vesicle, Plasma Membrane, Spindle Midzone.
Calculated MW:
24kDa
Observed MW:
24kDa
The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. [provided by RefSeq, Apr 2013]
Purification Method
Affinity purification
Gene ID
998 5879
RRID
AB_2863904
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from A-431 cells, using Phospho-Rac1/Cdc42-S71 Rabbit mAb (CABP1018) at 1:1000 dilution. A-431 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 3min.
