The RET Polyclonal Antibody is a valuable tool for researchers studying the RET protein, a receptor tyrosine kinase involved in the development and function of the nervous system, as well as cell growth and differentiation. This antibody, generated in rabbits, exhibits high specificity and sensitivity when detecting RET protein in human samples, making it an essential tool for Western blot applications.The RET protein is known to play a crucial role in various physiological processes, including cell survival, migration, and proliferation. Dysregulation of RET signaling has been implicated in the development of multiple diseases, including cancers such as medullary thyroid carcinoma and neuroblastoma.
By targeting the RET protein with this antibody, researchers can gain insights into the molecular mechanisms underlying these diseases and potentially identify new therapeutic targets.With its ability to specifically bind to the RET protein, the RET Polyclonal Antibody is a valuable asset for studies in cancer research, neuroscience, and developmental biology. By enabling the detection and analysis of RET in various cell types, this antibody facilitates a deeper understanding of the role of RET in health and disease, paving the way for the development of novel treatments and interventions.
Product Name:
Ret Polyclonal Antibody
SKU:
CAB0018
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1000-1100 of human Ret (NP_066124.1).
Sequence:
DISK DLEK MMVK RRDY LDLA ASTP SDSL IYDD GLSE EETP LVDC NNAP LPRA LPST WIEN KLYG MSDP NWPG ESPV PLTR ADGT NTGF PRYP NDSV YANW M
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200
Synonyms:
PTC; MTC1; HSCR1; MEN2A; MEN2B; CDHF12; CDHR16; RET-ELE1; Ret
Positive Sample:
Rat brain
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Endosome membrane, Single-pass type I membrane protein.
Calculated MW:
124kDa
Observed MW:
150kDa
This gene encodes a transmembrane receptor and member of the tyrosine protein kinase family of proteins. Binding of ligands such as GDNF (glial cell-line derived neurotrophic factor) and other related proteins to the encoded receptor stimulates receptor dimerization and activation of downstream signaling pathways that play a role in cell differentiation, growth, migration and survival. The encoded receptor is important in development of the nervous system, and the development of organs and tissues derived from the neural crest. This proto-oncogene can undergo oncogenic activation through both cytogenetic rearrangement and activating point mutations. Mutations in this gene are associated with Hirschsprung disease and central hypoventilation syndrome and have been identified in patients with renal agenesis.
Purification Method:
Affinity purification
Gene ID:
5979
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat brain, using Ret Rabbit pAb (CAB0018) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
