The Phospho-Smad2-S465/S467 + Smad3-S423/S425 Monoclonal Antibody (CABP1343) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated using advanced monoclonal antibody technology, specifically targets phosphorylated Smad2 at serine residues 465 and 467, as well as phosphorylated Smad3 at serine residues 423 and 425.One of the key functions of the Smad signaling pathway is its involvement in TGF-β signaling, a crucial pathway in regulating cell proliferation, differentiation, and apoptosis. By targeting phosphorylated Smad2 and Smad3, this antibody enables the detection and analysis of active Smad proteins in various cell types and tissues, providing valuable insights into their role in cellular processes.
This antibody is validated for use in - applications and has demonstrated reactivity against - samples.
Western blot analysis of various lysates, using Phospho-Smad2-S465/S467 + Smad3-S423/S425 Rabbit mAb (CABP1343) at1:2000 dilution. HeLa and NIH/3T3 cells were treated with TGF-β3（H） (200 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of various lysates using Phospho-Smad2-S465/S467 + Smad3-S423/S425 Rabbit mAb (CABP1343) at 1:1000 dilution. C6 cells were treated with TGF-β3 (200 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
