The Phospho-Smad2-S465/S467 + Smad3-S423/S425 Monoclonal Antibody (CABP1343) is a high-quality antibody developed for reliable detection and analysis of target proteins. -
This antibody is validated for use in - applications and has demonstrated reactivity against - samples.
Western blot analysis of various lysates, using Phospho-Smad2-S465/S467 + Smad3-S423/S425 Rabbit mAb (CABP1343) at1:2000 dilution. HeLa and NIH/3T3 cells were treated with TGF-β3（H） (200 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of various lysates using Phospho-Smad2-S465/S467 + Smad3-S423/S425 Rabbit mAb (CABP1343) at 1:1000 dilution. C6 cells were treated with TGF-β3 (200 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
