The Phospho-SRC Family-Y416 Monoclonal Antibody (CABP1370) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated by AssayGenie, specifically detects the phosphorylation of tyrosine 416 on Src family kinases, a critical event in their activation.The antibody is highly specific for phosphorylated Src kinases and has been validated for use in various applications, including Western blotting and immunofluorescence. Its high sensitivity and specificity make it ideal for studying the activation status of Src kinases in cell signaling pathways, leading to a better understanding of their role in cancer, inflammation, and other disease processes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Rat samples.
Product Name:
Phospho-SRC Family-Y416 Monoclonal Antibody
SKU:
CABP1370
Size:
20μL, 100μL
Reactivity:
Rat
Clone Number:
ARC57736
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
NEYT AR
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ASV, SRC1, THC6, c-SRC, p60-Src, SRC, Phospho-SRC Family-Y416
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Purification Method
Affinity purification
Gene ID
2534 3055 3932 4067 6714 7525
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells using Phospho-SRC Family-Y416 Rabbit mAb (CABP1370) at 1:1000 dilution. C6 cells were treated with Pervanadate (0.5 mM) at 37℃ for 40 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
