The Phospho-Src-Y529 Monoclonal Antibody (CABP1377) is a high-quality antibody developed for reliable detection and analysis of target proteins. Phosphorylation of this site is known to regulate the activity of Src, a key player in cell proliferation, differentiation, and survival.This antibody, generated using high-quality hybridoma technology, specifically targets the phosphorylated Y529 residue of Src tyrosine kinase, allowing for precise detection and analysis in various experimental settings. It is validated for use in Western blotting applications and has been shown to exhibit high specificity and sensitivity when used with human samples.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-Src-Y529 Monoclonal Antibody
SKU:
CABP1377
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC54665
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:2000 - 1:10000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Purification Method
Affinity purification
Gene ID
6714
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from A-431 cells, using Phospho-Src-Y529 Rabbit mAb (CABP1377) at 1:10000 dilution. A-431 cells were treated with EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
