The Phospho-STK11-S334 Antibody (CABP0601) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, has high specificity for human samples and has been validated for use in Western blot applications.STK11, also known as LKB1, is a tumor suppressor protein that plays a key role in regulating cell growth and metabolism. Phosphorylation of STK11 at serine 334 is known to impact its functions and influence downstream signaling pathways.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-STK11-S334 Antibody
SKU:
CABP0601
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
WRSM T
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PJS, LKB1, hLKB1, Phospho-STK11-S334
Positive Sample:
HeLa treated with PMA, HeLa treated with UV and PMA
Cellular Localization:
Cytoplasm, Membrane, Mitochondrion, Nucleus.
Calculated MW:
49kDa
Observed MW:
54kDa
The protein encoded by this gene is a serine/threonine kinase that regulates cell polarity and energy metabolism and functions as a tumor suppressor. Mutations in this gene have been associated with the autosomal dominant Peutz-Jeghers syndrome, as well as with skin, pancreatic, and testicular cancers.
Purification Method
Affinity purification
Gene ID
6794
RRID
AB_2771577
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-STK11-S334 Rabbit pAb (CABP0601) at 1:1000 dilution. Hela cells were treated with UV at room temperature for 30 minutes and PMA (200 nM) at 37℃ for 30 minutes after serum-starvation Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
