The Phospho-Syk-Y525/526 Antibody (CABP0542) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific for detecting phosphorylated Syk at tyrosine residues 525 and 526 in human samples, making it an essential reagent for Western blotting and other immunological assays.Syk is a critical enzyme involved in B cell receptor signaling, T cell receptor signaling, and other immune cell activation pathways. Phosphorylation at Y525/526 is known to regulate Syk activity, leading to downstream signaling events that impact immune responses.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-Syk-Y525/526 Antibody
SKU:
CABP0542
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
ENYY KA
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
IMD82, p72-Syk, Phospho-Syk-Y525/526
Positive Sample:
Jurkat
Cellular Localization:
Cell Membrane, Cytoplasm, Cytosol.
Calculated MW:
72kDa
Observed MW:
72kDa
This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
6850
RRID
AB_2771589
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Jurkat cells, using Phospho-Syk-Y525/526 Rabbit pAb (CABP0542) at 1:1000 dilution. Jurkat cells were treated by 10% FBS for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA.
