The TP53BP1 Polyclonal Antibody (CAB21632) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is highly specific and reactive with human samples, making it a valuable tool for investigating TP53BP1 expression and function in various cell types and tissues.TP53BP1, also known as tumor protein p53 binding protein 1, plays a critical role in maintaining genomic stability and cell cycle regulation. Dysregulation of TP53BP1 has been associated with various human diseases, including cancer and neurodevelopmental disorders.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TP53BP1 Polyclonal Antibody
SKU:
CAB21632
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
FCES SSET PFHF TLPK EGDI IPPL TGAT PPLI GHLK LEPK RHST PIGI SNYP ESTI ATSD VMSE SMVE THDP ILGS GKGD SGAA PDVD DKLC LRMK LVSP ETEA SEES LQFN LEKP ATGE RKNG STAV AESV ASPQ K
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:1000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
p202, 53BP1, TDRD30, p53BP1, TP53BP1
Positive Sample:
Mouse brain, Rat uterus
Cellular Localization:
Chromosome, Nucleus, Centromere, Kinetochore.
Calculated MW:
214kDa
Observed MW:
300-450kDa
This gene encodes a protein that functions in the DNA double-strand break repair pathway choice, promoting non-homologous end joining (NHEJ) pathways, and limiting homologous recombination. This protein plays multiple roles in the DNA damage response, including promoting checkpoint signaling following DNA damage, acting as a scaffold for recruitment of DNA damage response proteins to damaged chromatin, and promoting NHEJ pathways by limiting end resection following a double-strand break. These roles are also important during V(D)J recombination, class switch recombination and at unprotected telomeres. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method
Affinity purification
Gene ID
7158
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using TP53BP1 Rabbit pAb (CAB21632) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded Human brain using 53BP1 Rabbit pAb (CAB21632) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
