The Phospho-VASP-S157 Monoclonal Antibody (CABP1510) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed for use in various applications, including Western blot and immunofluorescence, specifically detects the phosphorylated form of VASP at serine 157. By targeting this specific phosphorylation site, researchers can gain insights into the regulation of VASP activity and its role in cellular processes. The antibody, produced using high-quality monoclonal antibodies, provides reliable and consistent results when used in experiments with human samples.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
Phospho-VASP-S157 Monoclonal Antibody
SKU:
CABP1510
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Clone Number:
ARC64203
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
RRVS NA
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:2000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
VASP, Phospho-VASP-S157
Positive Sample:
NIH/3T3 treated with Forskolin, C6 treated with Forskolin
Cellular Localization:
Cytoplasm, Cytoskeleton.
Calculated MW:
40kDa
Observed MW:
50kDa
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.
Purification Method
Affinity purification
Gene ID
7408
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from C6 cells, using Phospho-VASP-S157 Rabbit mAb (CABP1510) at 1:4000 dilution. C6 cells were treated with Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-VASP-S157 Rabbit mAb (CABP1510) at 1:4000 dilution. NIH/3T3 cells were treated with Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
