The Phospho-VEGF Receptor 2 (Y1175) Monoclonal Antibody is a cutting-edge tool for researchers studying VEGF receptor signaling pathways. This antibody, produced through monoclonal antibody technology, specifically targets the phosphorylated form of VEGF receptor 2 at the Y1175 site, which plays a key role in angiogenesis and tumor growth.The antibody is highly specific for human samples and has been rigorously validated for use in various applications, including Western blotting and immunohistochemistry. By binding to the phosphorylated form of VEGF receptor 2, researchers can easily detect and analyze the activation status of this important signaling molecule in different cell types and tissues.
VEGF receptor 2 is a critical regulator of blood vessel formation and is involved in a wide range of physiological and pathological processes, including cancer progression. Understanding the phosphorylation status of VEGF receptor 2 at the Y1175 site is essential for unraveling the complexities of angiogenesis and developing new strategies for targeting this pathway in cancer therapy. This monoclonal antibody provides a powerful tool for advancing research in the fields of oncology and vascular biology.
Product Name:
Phospho-VEGF Receptor 2-Y1175 Monoclonal Antibody
SKU:
CABP1385
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around Y1175 of human VEGF Receptor 2.
Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas.
Purification Method:
Affinity purification
Gene ID:
3791
Clone Number:
ARC57293
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of A-431 lysates, using Phospho-VEGF Receptor 2-Y1175 antibody (CABP1385) at 1:3000 dilution.A-431 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
