The Phospho-Vimentin-S56 Antibody (CABP0804) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including Western blot and immunofluorescence.Vimentin, a type III intermediate filament protein, is an essential component of the cytoskeleton and is involved in maintaining cellular structure and functionality. Phosphorylation of Vimentin at S56 is known to regulate its functions and is implicated in processes such as cell motility and cancer metastasis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
Phospho-Vimentin-S56 Antibody
SKU:
CABP0804
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
ASSP G
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.
Purification Method
Affinity purification
Gene ID
7431
RRID
AB_2771646
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-Vimentin-S56 pAb (CABP0804) at 1:3000 dilution or Vimentin antibody (CAB11952). HeLa cells were treated with Hydroxyurea (4 mM) at 37℃ for 20 hours.HeLa cells were treated with Paclitaxel (100 nM/mL) at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
