The Phospho-Vimentin-S83 Antibody (CABP1119) is a high-quality antibody developed for reliable detection and analysis of target proteins. Vimentin is a key intermediate filament protein involved in maintaining cell shape and structure. This antibody, raised in rabbits, is highly sensitive and specific for detecting phosphorylated vimentin at serine 83 in human samples.Phosphorylation of vimentin at serine 83 has been linked to various cellular processes, including cell migration, invasion, and signaling pathways. This antibody is validated for use in Western blot applications, enabling researchers to study the role of phosphorylated vimentin in different cell types and biological contexts.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-Vimentin-S83 Antibody
SKU:
CABP1119
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
QDSV D
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
HeLa treated with Paclitaxel, HeLa treated with Hydroxyurea, NIH/3T3 treated with Paclitaxel, NIH/3T3 treated with Hydroxyurea , C6 treated with Paclitaxel
Cellular Localization:
Cytoplasm.
Calculated MW:
54kDa
Observed MW:
57kDa
This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.
Purification Method
Affinity purification
Gene ID
7431
RRID
AB_2863988
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Vimentin-S83 Rabbit pAb (CABP1119) at 1:1000 dilution. NIH/3T3 cells were treated with Paclitaxel (100 nM/ml) at 37℃ for 20 hours. NIH/3T3 cells were treated with Hydroxyurea (4 mM) at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of lysates from C6 cells, using Phospho-Vimentin-S83 Rabbit pAb (CABP1119) at 1:1000 dilution. C6 cells were treated with Paclitaxel (100 nM) at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
