The Phospho-ZAP70 (Y319) Polyclonal Antibody (CABP0467) is a highly specific antibody designed for research involving the phosphorylation of ZAP70 at tyrosine 319. ZAP70 is a cytoplasmic protein tyrosine kinase that plays a critical role in T-cell receptor signaling and immune response activation. The phosphorylation of ZAP70 at Y319 is a key event in T-cell activation and signaling pathways.This antibody, raised in rabbits, is validated for use in various applications, including Western blotting, immunoprecipitation, and immunofluorescence. It specifically recognizes the phosphorylated form of ZAP70 at Y319, enabling the detection and analysis of ZAP70 activation in cell lysates and tissues.
Research involving ZAP70 phosphorylation at Y319 is important for understanding T-cell signaling and activation in immune responses. Dysregulation of ZAP70 phosphorylation has been implicated in various diseases, including autoimmune disorders and cancers. Therefore, this antibody is valuable for studies in immunology, cancer research, and other fields aimed at elucidating the role of ZAP70 in health and disease.
Product Name:
Phospho-ZAP70-Y319 Rabbit Polyclonal Antibody
SKU:
CABP0467
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around Y319 of human ZAP70 (NP_001070.2).
Sequence:
SPYS D
Tested Applications:
WBIPELISA
Recommended Dilution:
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
7535
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Immunoprecipitation analysis of 200 μg extracts of Jurkat cells, using 3 μg Phospho-ZAP70-Y319 pAb (CABP0467). Western blot was performed from the immunoprecipitate using Phospho-ZAP70-Y319 pAb (CABP0467) at a dilution of 1:1000. Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37℃ for 2 minutes after serum-starvation overnight.
