The Phospho-ZAP70-Y493 Monoclonal Antibody (CABP1435) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using advanced monoclonal technology, specifically targets the phosphorylated tyrosine 493 residue on ZAP70, enabling precise detection and analysis in a variety of experimental settings.Phosphorylation of ZAP70 at tyrosine 493 is crucial for initiating downstream signaling cascades that regulate T cell activation and effector functions. By targeting this specific phosphorylation site, researchers can gain insights into the intricate molecular mechanisms underlying immune responses and immune cell signaling pathways.
This antibody is validated for use in IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-ZAP70-Y493 Monoclonal Antibody
SKU:
CABP1435
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC59392
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
SYYT A
Tested Applications:
IF/ICCELISA
Recommended Dilution:
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
7535
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Immunofluorescence analysis of Jurkat treated with H2O2 and Jurkat cells using Phospho-ZAP70-Y493 Rabbit mAb (CABP1435) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
