The PHOX2B Monoclonal Antibody (CAB6816) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is validated for use in various applications such as immunohistochemistry and Western blotting, enabling detection and analysis of Phox2B in different cell types and tissues.Phox2B is known for its crucial role in controlling the development of neurons involved in the autonomic nervous system, making it a key player in regulating basic physiological functions such as breathing, heart rate, and digestion.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
PHOX2B Monoclonal Antibody
SKU:
CAB6816
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Clone Number:
ARC1420
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CCHS, PMX2B, NBLST2, NBPhox, PHOX2B
Positive Sample:
SH-SY5Y, Neuro-2a
Cellular Localization:
Nucleus.
Calculated MW:
32kDa
Observed MW:
35kDa
The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription factor involved in the development of several major noradrenergic neuron populations and the determination of neurotransmitter phenotype. The gene product is linked to enhancement of second messenger-mediated activation of the dopamine beta-hydroylase, c-fos promoters and several enhancers, including cyclic amp-response element and serum-response element. Expansion of a 20 amino acid polyalanine tract in this protein by 5-13 aa has been associated with congenital central hypoventilation syndrome.
Purification Method
Affinity purification
Gene ID
8929
RRID
AB_2863538
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PHOX2B Rabbit mAb (CAB6816) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
