The PIK3R3/p55PIK Monoclonal Antibody (CAB3980) is a high-quality antibody developed for reliable detection and analysis of target proteins. Phosphatidylinositol 3-kinase (PI3K) phosphorylates phosphatidylinositol and similar compounds, which then serve as second messengers in growth signaling pathways. PI3K is composed of a catalytic and a regulatory subunit. The protein encoded by this gene represents a regulatory subunit of PI3K. The encoded protein contains two SH2 domains through which it binds activated protein tyrosine kinases to regulate their activity. RRID AB_2863169 Gene ID 8503 Swiss Prot Synonym p55; p55PIK; p55-GAMMA; PIK3R3/p55PIK
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PIK3R3/p55PIK Monoclonal Antibody
SKU:
CAB3980
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0320
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
p55, p55PIK, p55-GAMMA, PIK3R3/p55PIK
Positive Sample:
PC-3, Raji, SH-SY5Y, Mouse testis, Mouse brain, Rat testis, Rat brain
Cellular Localization:
Cytosol.
Calculated MW:
54kDa
Observed MW:
52kDa
Phosphatidylinositol 3-kinase (PI3K) phosphorylates phosphatidylinositol and similar compounds, which then serve as second messengers in growth signaling pathways. PI3K is composed of a catalytic and a regulatory subunit. The protein encoded by this gene represents a regulatory subunit of PI3K. The encoded protein contains two SH2 domains through which it binds activated protein tyrosine kinases to regulate their activity. RRID AB_2863169 Gene ID 8503 Swiss Prot Synonym p55; p55PIK; p55-GAMMA; PIK3R3/p55PIK
Purification Method:
Affinity purification
Gene ID:
8503
RRID:
AB_2863169
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PIK3R3/p55PIK Rabbit mAb (CAB3980) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
