The PI3 Kinase p85 beta Monoclonal Antibody (CAB4860) is a high-quality antibody developed for reliable detection and analysis of target proteins. Phosphatidylinositol 3-kinase (PI3K) is a lipid kinase that phosphorylates phosphatidylinositol and similar compounds, creating second messengers important in growth signaling pathways. PI3K functions as a heterodimer of a regulatory and a catalytic subunit. The protein encoded by this gene is a regulatory component of PI3K. Three transcript variants, one protein coding and the other two non-protein coding, have been found for this gene. RRID AB_2863364 Gene ID 5296 Swiss Prot Synonym p85; MPPH; P85B; MPPH1; p85beta; p85-BETA; PI3 Kinase p85 beta
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
PI3 Kinase p85 beta Monoclonal Antibody
SKU:
CAB4860
Size:
100μL, 20μL
Reactivity:
Human, Rat
Clone Number:
ARC0287
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Phosphatidylinositol 3-kinase (PI3K) is a lipid kinase that phosphorylates phosphatidylinositol and similar compounds, creating second messengers important in growth signaling pathways. PI3K functions as a heterodimer of a regulatory and a catalytic subunit. The protein encoded by this gene is a regulatory component of PI3K. Three transcript variants, one protein coding and the other two non-protein coding, have been found for this gene. RRID AB_2863364 Gene ID 5296 Swiss Prot Synonym p85; MPPH; P85B; MPPH1; p85beta; p85-BETA; PI3 Kinase p85 beta
Purification Method:
Affinity purification
Gene ID:
5296
RRID:
AB_2863364
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PI3 Kinase p85 beta Rabbit mAb (CAB4860) at 1:1000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
