The PI3 Antibody (CAB5441) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and shows high reactivity with human samples, making it suitable for use in Western blot applications. By targeting the Elafin protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential component for studies in immunology and inflammatory diseases.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
PI3 Antibody
SKU:
CAB5441
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ESI, WAP3, SKALP, WFDC14, cementoin, PI3
Positive Sample:
U-87MG, HepG2
Cellular Localization:
Secreted.
Calculated MW:
12kDa
Observed MW:
12kDa
This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria, and fungal pathogens. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines.
Purification Method
Affinity purification
Gene ID
5266
RRID
AB_2766243
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PI3 Rabbit pAb (CAB5441) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry analysis of paraffin-embedded Human colon using PI3 Rabbit pAb (CAB5441) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
