The PLA2G4E Polyclonal Antibody (PACO22299) is a valuable tool for researchers studying PLA2G4E, an enzyme involved in the metabolism of phospholipids and the production of lipid mediators. This antibody, produced in rabbits, has high reactivity with human samples and is specifically validated for use in Western blot applications. By binding to the PLA2G4E protein, this antibody allows for accurate detection and analysis in a variety of cell types, making it an ideal choice for studies in lipid metabolism, inflammation, and cancer research.PLA2G4E, also known as cytosolic phospholipase A2 epsilon, plays a crucial role in inflammatory responses and lipid signaling pathways.
Its involvement in the generation of lipid mediators makes it a key player in processes like inflammation, immune responses, and tumorigenesis. Research into the function and regulation of PLA2G4E is essential for understanding its contribution to diseases such as cancer, cardiovascular disorders, and neurological conditions. This antibody provides a reliable tool for investigating the role of PLA2G4E in these contexts and developing potential therapeutic strategies.
Antibody Name:
PLA2G4E Antibody (PACO22299)
Antibody SKU:
PACO22299
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:50-1:100
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from internal of human PLA2G4E.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using PLA2G4E antibody.
Background:
Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position By similarity.
PLA2G4E: a calcium-dependent phospholipase A2 that catalyzes the release of arachidonic acid from membrane phospholipids. Selectively hydrolyzes glycerophospholipids in the sn-2 position. Translocates to lysosomal membranes in a calcium-dependent fashion. The N-terminal C2 domain associates with lipid membranes and mediates its regulation by presenting the active site to its substrate in response to elevations of cytosolic Ca2+. Two isoforms of the human protein are produced by alternative splicing.Protein type: Lipid Metabolism - alpha-linolenic acid; Phospholipase; Lipid Metabolism - arachidonic acid; Lipid Metabolism - linoleic acid; Lipid Metabolism - ether lipid; Lipid Metabolism - glycerophospholipid; EC 3.1.1.4Chromosomal Location of Human Ortholog: 15q15.1Cellular Component: lysosomal membrane; cytosolMolecular Function: phospholipase A2 activity; metal ion bindingBiological Process: phospholipid catabolic process; phospholipid metabolic process; glycerophospholipid biosynthetic process
