The PLC beta 3 (PLCB3) Antibody (CAB10236) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Alternative splicing results in multiple transcript variants. RRID AB_2757761 Gene ID 5331 Swiss Prot Synonym SMDCD; PLC beta 3 (PLCB3)
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PLC beta 3 (PLCB3) Antibody
SKU:
CAB10236
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
SMDCD, PLC beta 3 (PLCB3)
Positive Sample:
OVCAR8, Mouse lung, Rat lung
Cellular Localization:
Membrane, Peripheral Membrane Protein.
Calculated MW:
139kDa
Observed MW:
160kDa
This gene encodes a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Alternative splicing results in multiple transcript variants. RRID AB_2757761 Gene ID 5331 Swiss Prot Synonym SMDCD; PLC beta 3 (PLCB3)
Purification Method:
Affinity purification
Gene ID:
5331
RRID:
AB_2757761
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PLC beta 3 (PLCB3) Rabbit pAb (CAB10236) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Rat brain using PLC beta 3 (PLC beta 3 (PLCB3)) Rabbit pAb (CAB10236) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
