The Anti-Pmel Antibody (CAB5384) is a highly specific and sensitive tool for researchers studying melanosome biology and melanoma progression. This polyclonal antibody, generated in rabbits, targets the Pmel protein and is suitable for use in techniques such as immunofluorescence, immunoprecipitation, and flow cytometry. Its high reactivity with human samples ensures reliable results in a variety of experimental settings.Pmel, also known as gp100, is a key component of melanosome formation and plays a crucial role in melanin synthesis. Dysregulation of Pmel expression is associated with melanoma, a highly aggressive form of skin cancer.
By targeting Pmel, researchers can gain valuable insights into the molecular mechanisms underlying melanoma development and progression.Using the Anti-Pmel Antibody allows for accurate detection and analysis of Pmel protein levels in various cell types, making it an indispensable tool for studies in cancer biology and melanoma research. Understanding the role of Pmel in melanosome biology and melanoma pathogenesis is essential for the development of novel therapeutic strategies for the treatment of this deadly disease.
Product Name:
PMEL Rabbit Polyclonal Antibody
SKU:
CAB5384
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 320-430 of human PMEL (NP_008859.1).
Endoplasmic reticulum membrane, Endosome, Golgi apparatus, Melanosome, Secreted, Single-pass type I membrane protein, multivesicular body.
Calculated MW:
70kDa
Observed MW:
80kDa/105kDa
This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
6490
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from A375 cells, using PMEL Rabbit pAb (CAB5384) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
