The POLE Antibody (PACO4632) is a polyclonal antibody specifically designed for research involving the POLE protein, implicated in DNA replication and repair processes. This antibody, produced in rabbits, shows high reactivity with human samples and is validated for use in a variety of applications, including Western blotting and immunofluorescence.POLE, also known as DNA polymerase epsilon, plays a crucial role in maintaining genomic stability and fidelity during cell division. Mutations in the POLE gene have been associated with various cancers, highlighting its importance in tumorigenesis and cancer progression.
Research into the function and regulation of POLE is essential for developing targeted therapies and precision medicine approaches in cancer treatment.The POLE Antibody (PACO4632) enables researchers to detect and analyze the expression of the POLE protein in different cell types, making it a valuable tool for studies in cancer biology, genomics, and personalized medicine. By understanding the role of POLE in DNA replication and repair, scientists can uncover novel molecular targets for therapeutic intervention and improve patient outcomes in cancer treatment.
Antibody Name:
POLE Antibody
Antibody SKU:
PACO04632
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
IHC:1:100-1:300
Species Reactivity:
Human, Rat
Immunogen:
synthesized peptide derived from the Internal region of human DNA pol epsilon A.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Synonyms:
POLE; POLE1; DNA polymerase epsilon catalytic subunit A; DNA polymerase II subunit A
UniProt Protein Function:
POLE: Participates in DNA repair and in chromosomal DNA replication. Belongs to the DNA polymerase type-B family.Protein type: Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; EC 2.7.7.7; DNA replication; DNA repair, damage; TransferaseChromosomal Location of Human Ortholog: 12q24.3Cellular Component: nucleoplasm; epsilon DNA polymerase complex; cytoplasm; plasma membrane; nucleusMolecular Function: DNA binding; zinc ion binding; 4 iron, 4 sulfur cluster binding; nucleotide binding; DNA-directed DNA polymerase activity; chromatin bindingBiological Process: DNA replication initiation; in utero embryonic development; DNA repair; base-excision repair, gap-filling; telomere maintenance via semi-conservative replication; DNA synthesis during DNA repair; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; DNA replication; telomere maintenance; G1/S transition of mitotic cell cycleDisease: Colorectal Cancer, Susceptibility To, 12; Facial Dysmorphism, Immunodeficiency, Livedo, And Short Stature
UniProt Protein Details:
NCBI Summary:
This gene encodes the catalytic subunit of DNA polymerase epsilon. The enzyme is involved in DNA repair and chromosomal DNA replication. Mutations in this gene have been associated with colorectal cancer 12 and facial dysmorphism, immunodeficiency, livedo, and short stature. [provided by RefSeq, Sep 2013]
