The Porcine Cathepsin L (CTSL) ELISA Kit is specifically designed for the accurate and reliable measurement of Cathepsin L levels in porcine serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing researchers with precise and reproducible results for a variety of research applications.Cathepsin L is a key enzyme involved in various biological processes, including protein degradation, antigen processing, and tissue remodeling. Dysregulation of Cathepsin L has been linked to numerous diseases such as cancer, osteoporosis, and cardiovascular disorders, highlighting its importance as a potential therapeutic target and biomarker for disease diagnosis and monitoring.
With the Porcine Cathepsin L ELISA Kit, researchers can accurately quantify Cathepsin L levels in porcine samples, enabling better understanding of its role in disease progression and the development of innovative treatment strategies. Suiting a wide range of research needs, this kit is a valuable tool for investigating the intricate mechanisms underlying Cathepsin L function and its implications in various pathologies.
Product Name:
Porcine Cathepsin L (CTSL) ELISA Kit
Product Code:
PRDL00035
Size:
96 Assays
Target:
CTSL
Synonyms:
CTSL1, CATL, CTS-L, MEP, Cathepsin L1, Major excreted protein
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
