SynonymsSLC11A2,DCT1,NRAMP2,Natural resistance-associated macrophage protein 2,Solute Carrier Family 11 Member 2,Proton-Coupled Divalent Metal Ion Transporters
ReactivityPorcine
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
SKU:
PRDL00041
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
B2ZDZ3
Sensitivity:
0.062ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
SLC11A2,DCT1,NRAMP2,Natural resistance-associated macrophage protein 2,Solute Carrier Family 11 Member 2,Proton-Coupled Divalent Metal Ion Transporters
Reactivity:
Porcine
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
Porcine Divalent Metal Transporter 1 (DMT1) ELISA Kit
The Porcine Divalent Metal Transporter 1 (DMT1) ELISA Kit is specifically designed for the quantitative measurement of DMT1 levels in porcine serum, plasma, and tissue homogenates. This kit offers exceptional accuracy and precision, guaranteeing dependable and consistent results for various research purposes.DMT1 is a key protein responsible for the transport of divalent metals such as iron across cellular membranes.
Its dysregulation has been linked to disorders like anemia, neurodegenerative diseases, and iron overload conditions. By detecting and quantifying DMT1 levels, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially develop targeted interventions for their management.
Product Name:
Porcine Divalent Metal Transporter 1 (DMT1) ELISA Kit
Product Code:
PRDL00041
Size:
96 Assays
Target:
DMT1
Synonyms:
SLC11A2, DCT1, NRAMP2, Natural resistance-associated macrophage protein 2, Solute Carrier Family 11 Member 2,Proton-Coupled Divalent Metal Ion Transporters
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
