The Porcine INS (Insulin) ELISA Kit is a specialized assay designed for the precise quantitative determination of Insulin levels in porcine biological samples. Insulin, a pivotal hormone produced by the pancreas, plays a critical role in regulating blood glucose levels and facilitating cellular glucose uptake in pigs.
Monitoring Insulin concentrations aids in assessing metabolic balance, glucose metabolism, and pancreatic function in porcine physiology. This ELISA kit provides a reliable and accurate method for quantifying Insulin levels, enabling researchers to investigate glucose homeostasis, insulin sensitivity, and metabolic health in porcine models. By measuring Insulin concentrations in porcine samples, researchers can explore insulin secretion, pancreatic health, and metabolic disturbances related to diabetes and metabolic disorders in pigs. With exceptional sensitivity and specificity, the Porcine INS ELISA Kit ensures precise and reproducible results, making it a valuable tool for studies focused on porcine metabolic regulation and endocrine function. Manufactured under strict quality control standards, this kit offers robust performance and user-friendly protocols, facilitating comprehensive research into porcine metabolism and endocrine physiology.
Product Name:
Porcine INS (Insulin) ELISA Kit
SKU:
AEES00692
Target:
Porcine INS (Insulin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine INS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine INS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine INS. You can calculate the concentration of Porcine INS in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-96
88-101
98-113
Average (%)
91
95
105
1:4
Range (%)
86-101
84-96
99-113
Average (%)
93
90
107
1:8
Range (%)
82-94
86-97
97-112
Average (%)
89
91
105
1:16
Range (%)
83-95
90-102
98-110
Average (%)
89
95
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-100
94
EDTA plasma (n=5)
94-106
101
Cell culture media (n=5)
86-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.06
2.6
9.64
1.06
2.82
8.85
Standard deviation
0.07
0.11
0.48
0.06
0.13
0.41
C V (%)
6.6
4.23
4.98
5.66
4.61
4.63
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Porcine INS concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Porcine INS in samples. No significant cross-reactivity or interference between Porcine INS and analogues was observed.
