The Porcine Interleukin-13 (IL-13) ELISA Kit offered by Assay Genie is a valuable tool for the quantitative measurement of IL-13 levels in porcine serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers accurate and consistent results, making it suitable for a variety of research applications.IL-13 is a key cytokine involved in the regulation of immune responses, particularly in allergic and inflammatory reactions. Its dysregulation has been implicated in various diseases, including asthma, allergies, and autoimmune disorders.
By accurately measuring IL-13 levels, researchers can gain valuable insights into the underlying mechanisms of these conditions and potentially identify novel therapeutic targets.Overall, the Porcine IL-13 ELISA Kit from Assay Genie provides researchers with a reliable and efficient tool for studying the role of IL-13 in porcine health and disease, making it an indispensable addition to any laboratory toolkit.
Product Name:
Porcine Interleukin 13 (IL-13) ELISA Kit
Product Code:
PRDL00083
Size:
96 Assays
Target:
IL13
Synonyms:
ALRH, BHR1, P600, IL-13, NC30
Detection Method:
Sandwich
Range:
15.625-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
