The Porcine NPY (Neuropeptide Y) ELISA Kit is a specialized assay designed for the quantitative measurement of neuropeptide Y levels in porcine biological samples. Neuropeptide Y (NPY) is a critical neurotransmitter known for its regulatory functions in various physiological processes, including appetite regulation, stress response, cardiovascular function, and energy balance. This ELISA kit enables accurate and sensitive detection of NPY concentrations in porcine samples, providing valuable insights into the neuroendocrine system and the role of NPY in porcine physiology. NPY acts as a potent appetite stimulant and plays a significant role in the central regulation of feeding behavior and energy homeostasis in pigs.
With exceptional sensitivity and specificity, the Porcine NPY ELISA Kit offers reliable and reproducible results, making it a valuable tool for studying the intricate neuroendocrine pathways and metabolic processes regulated by NPY in porcine models. Manufactured under stringent quality control standards, this kit ensures robust performance and ease of use, making it an excellent choice for research in porcine neurobiology and metabolic studies.
Product Name:
Porcine NPY (Neuropeptide Y) ELISA Kit
SKU:
AEES00707
Target:
Porcine NPY (Neuropeptide Y)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with NPY. During the reaction, NPY in the sample or standard competes with a fixed amount of NPY on the solid phase supporter for sites on the Biotinylated Detection Ab specific to NPY. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NPY in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
88-104
95-109
Average (%)
93
95
103
1:4
Range (%)
87-102
85-98
96-110
Average (%)
93
90
103
1:8
Range (%)
86-98
92-104
97-112
Average (%)
93
97
102
1:16
Range (%)
90-101
90-103
98-115
Average (%)
95
96
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
90
EDTA plasma (n=5)
85-98
92
Cell culture media (n=5)
90-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
92.87
271.37
937.55
97.88
275.68
1003.8
Standard deviation
5.89
12.51
42.38
5.03
14.45
46.38
C V (%)
6.34
4.61
4.52
5.14
5.24
4.62
Sample type &Sample volume:
; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of NPY concentrations in .
Specificity:
This kit recognizes NPY in samples. No significant cross-reactivity or interference between NPY and analogues was observed.
