Porcine Protein C Receptor, Endothelial (PROCR) ELISA Kit
The Porcine Protein C Receptor (Endothelial PROC) ELISA Kit offered by Assay Genie is specifically designed for the accurate detection of protein C receptor levels in porcine serum, plasma, and cell culture supernatants. This ELISA kit boasts high sensitivity and specificity, ensuring reliable and reproducible results for a wide range of research applications.Protein C receptor is a key player in the regulation of blood coagulation and inflammation, making it an important biomarker for studying various diseases and conditions. With its ability to promote blood vessel formation and influence cell proliferation, protein C receptor is implicated in conditions such as cardiovascular diseases, cancer, and neurodegenerative disorders.
By using the Porcine Protein C Receptor ELISA Kit, researchers can gain valuable insights into the role of protein C receptor in various physiological and pathological processes, ultimately aiding in the development of potential therapeutic strategies. Trust Assay Genie for accurate and reliable results in your research endeavors.
Product Name:
Porcine Protein C Receptor, Endothelial (PROCR) ELISA Kit
Product Code:
PRDL00129
Size:
96 Assays
Target:
PROCR
Synonyms:
CD201, CCCA , CCD41, BA42O4.2, EPCR, Endothelial Protein C Receptor, Cell Cycle, Centrosome-Associated Protein, Activated Protein C Receptor
Detection Method:
Sandwich
Range:
15.6-1,000ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
