BOB1 Polyclonal Antibody (CAB24457)
The BOB1 Polyclonal Antibody (CAB24457) is a high-quality antibody developed for reliable detection and analysis of target proteins. nan
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
| Product Name: | BOB1 Polyclonal Antibody |
| SKU: | CAB24457 |
| Size: | 100μL, 20μL |
| Reactivity: | Human |
| Conjugate: | Unconjugated |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | | WB | 1:500 - 1:1000 | | ELISA | Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. |
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| Synonyms: | POU2AF1, BOB1, OBF-1, OBF1, OCAB, POU class 2 associating factor 1 |
| Positive Sample: | Daudi |
| Cellular Localization: | Nucleus. |
| Calculated MW: | 27kDa |
| Observed MW: | 37kDa |
| Purification Method | Affinity purification |
| Gene ID | 5450 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3. |
 | Western blot analysis of lysates from Daudi cells, using BOB1 Rabbit pAb (CAB24457) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s. |
