The Pou5f1b Polyclonal Antibody (CAB15705) is a valuable tool for researchers studying the transcription factor Pou5f1b, also known as Oct-4. This antibody, raised in rabbits, exhibits high specificity and reactivity towards human samples, making it an excellent choice for Western blot applications.Pou5f1b/Oct-4 is a key player in the regulation of pluripotency and self-renewal in embryonic stem cells, making it an essential target for understanding cellular differentiation and development. Its role in maintaining stem cell populations and influencing gene expression patterns makes it a vital marker for identifying and studying stem cells in both research and clinical settings.
By utilizing the Pou5f1b Polyclonal Antibody, researchers can accurately detect and analyze Pou5f1b/Oct-4 protein levels in various cell types, facilitating insights into stem cell biology, regenerative medicine, and potential therapeutic applications. This antibody is a valuable tool for advancing our understanding of pluripotency and stem cell technologies with implications for tissue engineering, disease modeling, and personalized medicine.
Product Name:
POU5F1B Rabbit Polyclonal Antibody
SKU:
CAB15705
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human POU5F1B (NP_001153014.1).
This intronless gene was thought to be a transcribed pseudogene of POU class 5 homeobox 1, however, it has been reported that this gene can encode a functional protein. The encoded protein is nearly the same length as and highly similar to the POU class 5 homeobox 1 transcription factor, has been shown to be a weak transcriptional activator and may play a role in carcinogenesis and eye development.
Purification Method:
Affinity purification
Gene ID:
5462
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using POU5F1B Rabbit pAb (CAB15705) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
