The PP2A-B55alpha/PR55alpha/PPP2R2A Monoclonal Antibody (CAB24261) is a high-quality antibody developed for reliable detection and analysis of target proteins. The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants have been described. RRID Gene ID 5520 Swiss Prot Synonym B55A; PR52A; PR55A; B55ALPHA; PR55alpha; PP2A-B55α/PR55α/PPP2R2A
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants have been described. RRID Gene ID 5520 Swiss Prot Synonym B55A; PR52A; PR55A; B55ALPHA; PR55alpha; PP2A-B55α/PR55α/PPP2R2A
Purification Method:
Affinity purification
Gene ID:
5520
RRID:
-
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using PP2A-B55α/PR55α/PPP2R2A Rabbit mAb (CAB24261) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Western blot analysis of lysates from Mouse brain using PP2A-B55α/PR55α/PPP2R2A Rabbit mAb (CAB24261) at 1:2000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
